Reporter

Part:BBa_K137024:Design

Designed by: Allen Lin   Group: iGEM08_Caltech   (2008-07-20)

GFP with (A)24 repeat after start codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 668


Design Notes

With 24 A repeats, the GFP sequence is in frame with respect to the start codon, so a functional GFP should be transcribed.

Source

The nucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.

References